| 第 44 卷第 3 期 |  | Vol. 44 No. 3 | | 2014 年 6 月 | Jun 2014 |
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所屬欄目：分析與測試
高效液相色譜-熒光法測定阿維菌素在稻田環(huán)境基質(zhì)中的殘留 |
| 寧 贏(yíng)1，彭秧錫1，歐曉明1，2，3*，梁 驥2，3，張貴群2，3，劉義珂2，3，聶思橋2，3，任 競2，3，劉金勝2，3，傅 強2，3
（1. 湖南人文科技學(xué)院，湖南 婁底 417000；2. 湖南化工研究院 國家農藥創(chuàng )制工程技術(shù)研究中心，湖南 長(cháng)沙 410014；3. 農用化學(xué)品湖南省重點(diǎn)實(shí)驗室，湖南 長(cháng)沙 410014） |
| 摘 要：采用高效液相色譜-熒光檢測器（HPLC-FLD）建立了阿維菌素在水稻植株、谷殼、糙米、土壤及田水中的殘留檢測方法。樣品用乙腈提取，經(jīng)液液萃取凈化，濃縮定容后衍生化，HPLC-FLD檢測，外標法定量。結果表明，在添加水平為0.01～0.50 mg／kg時(shí)，阿維菌素在水稻植株、谷殼、糙米、土壤和田水中的平均添加回收率為79.13%～102.98%，相對標準偏差為3.10%～6.71%。該方法的最小檢出量為0.2 ng，在水稻植株、土壤、谷殼、糙米和田水中的最低檢測濃度均為0.01 mg／kg。 |
| 關(guān)鍵詞：高效液相色譜-熒光檢測器；阿維菌素；稻田；殘留 |
| 中圖分類(lèi)號：O657.7+1 文獻標識碼：A 文章編號：1009-9212（2014）03-0066-04 |
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| Determination of Abamectin Residues in Paddy Field Environmental Matrices by High Performance Liquid Chromatography with Fluorescence Detector |
| NING Ying1, PENG Yang-xi1, OU Xiao-ming1,2,3*, LIANG Ji2,3, ZHANG Gui-qun2,3, LIU Yi-ke2,3, NIE Si-qiao2,3, REN Jing2,3, LIU Jin-sheng2,3, FU Qiang2,3
(1. Hunan University of Humanities, Science and Technology, Loudi 417000, China; 2. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry, Changsha 410014, China; 3. Hunan Province Key Laboratory for Agrochemicals, Changsha 410014, China) |
| Abstract：A method for the determination of abamectin residue in paddy plants, husk, coarse rice, soil and water was established using high performance liquid chromatography with fluorescence detector(HPLC-FLD). The samples were extracted with acetonitrile, cleaned up by liquid-liquid separation, and directly pre-column derivatized after concentration and constant volume. The analysis was performed by HPLC-FLD with an external standard. The results showed that the average recoveries of abamectin in rice plants, husk, coarse rice, soil and water were from 79.10% to 102.98%, and the relative standard deviations were from 3.10% to 6.71% when the spiked levels ranged from 0.01 mg/kg to 0.50 mg/kg. The LOD was 0.2 ng, and the LOQ was 0.01 mg/kg in paddy plants, husk, rice, soil and water. |
| Key words：HPLC-FLD; abamectin; paddy field; residues |
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作者簡(jiǎn)介：寧 贏(yíng)（1990-），男，湖南衡陽(yáng)人，碩士研究生，研究方向：農藥殘留檢測（E-mail：1005861004@qq.com）。
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聯(lián) 系 人： 歐曉明，研究員，博士，研究方向：農藥殘留化學(xué)、環(huán)境毒理和農藥生物學(xué)（E-mail：xmouhn@163.com）。
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收稿日期：2014-05-16
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