| 第 44 卷第 5 期 |  | Vol. 44 No. 5 | | 2014 年 10 月 | Oct 2014 |
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所屬欄目：分析與測試
分散液液微萃取-氣相色譜分析尿碘含量 |
| 葉海朋，江 陽(yáng)，周 凱
（杭州市上城區疾病預防控制中心，浙江 杭州 310009） |
| 摘 要：以過(guò)硫酸銨消解處理尿液，用丁酮衍生后以二硫化碳為微萃取劑，甲醇為分散劑，用分散液液微萃取技術(shù)提取尿中碘代丁酮，以氣相色譜-電子捕獲檢測器分析，用外標法定量。尿碘的線(xiàn)性范圍為10~300 μg／L，相關(guān)系數r=0.999 5，檢出限0.20 μg／L；方法回收率達91.9%~97.7%，相對標準偏差為2.4%~z4.6%（n=6）。該方法操作簡(jiǎn)便、靈敏度高，適于尿碘批量快速分析。 |
| 關(guān)鍵詞：尿碘；分散液液微萃��；氣相色譜 |
| 中圖分類(lèi)號：O657.7+1 文獻標識碼：A 文章編號：1009-9212（2014）05-0070-03 |
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| Analysis of Iodine in Urine by Dispersive Liquid-liquid Micro-extraction and Gas Chromatography |
| YE Hai-peng, JIANG Yang, ZHOU Kai
(Shangcheng District Center for Disease Control and Prevention, Hangzhou 310009, China) |
| Abstract：To detect iodine in urine by dispersive liquid- liquid micro-extraction and gas chromatography, the urine was pre-treated with ammonium persulfate and 2-butanone, and then extracted with carbon disulfide and methanol. Iodobutanone in the extract was analyzed with GC- μECD and quantified by means of external standard curve. The detection limit of the iodine in urine was 0.20 μg/L, and the standard curve was linear in the range of 10~300 μg/L (r=0.9995). The average recovery rate of iodine was 91.9% ~97.7% and the relative standard deviation (RSD) was from 2.4% to 4.6% (n=6). Conclusion: This method is simple, highly sensitive and suitable for quick analysis of iodine in urine. |
| Key words：iodine urine; dispersive liquid-liquid micro-extraction; gas chromatography |
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基金項目：浙江省醫藥衛生科技計劃項目（2011KYB087）。
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作者簡(jiǎn)介： 葉海朋（1986-），男，安徽安慶人，檢驗技師，從事衛生檢驗工作。
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收稿日期：2014-09-25
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