| 第 45 卷第 2 期 |  | Vol. 45 No. 2 | | 2015 年 4 月 | Apr 2015 |
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所屬欄目：分析與測試
HPLC法測定N-羥基-N-（2-（（1-（4-氯苯基）-1H-吡唑-3-基）氧）甲基）苯基氨基甲酸甲酯 |
| 孔曉紅1，2，王艷麗1，2
（1. 湖南化工研究院 國家農藥創(chuàng )制工程技術(shù)研究中心，湖南 長(cháng)沙 410014；2. 農用化學(xué)品湖南省重點(diǎn)實(shí)驗室，湖南 長(cháng)沙 410014） |
| 摘 要：采用Aglient Extend-C18 5 μm 250 mm×4.6 mm色譜柱，流動(dòng)相為甲醇-0.4%磷酸溶液（v∶v=75∶25），流速為1 mL／min，275 nm為檢測波長(cháng)，建立了測定N-羥基-N-（2-（（1-（4-氯苯基）-1H-吡唑-3-基）氧）甲基）苯基氨基甲酸甲酯含量的高效液相色譜分析方法。在進(jìn)樣量為0.53～3.52 μg的范圍內線(xiàn)性關(guān)系良好（y=125679.4x+15010.4，r=0.9999），RSD 0.24%，回收率99.2%～100.4%，分析周期為15 min。方法快速、簡(jiǎn)便、準確，可用于N-羥基-N-（2-（（1-（4-氯苯基）-1H-吡唑-3-基）氧）甲基）苯基氨基甲酸甲酯反應液及產(chǎn)品的質(zhì)量控制 |
| 關(guān)鍵詞：N-羥基-N-（2-（（1-（4-氯苯基）-1H-吡唑-3-基）氧）甲基）苯基氨基甲酸甲酯；HPLC；測定 |
| 中圖分類(lèi)號：O657.7+2 文獻標識碼：A 文章編號：1009-9212（2015）02-0070-03 |
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| Determination of methyl 2-((1-(4-chlorophenyl)-1H-pyrazol-3-yloxy)methyl)phenyl(hydroxy)carbamate by HPLC |
| KONG Xiao-hong1, WANG Yan-li1
(1. Hunan Research Institute of Chemical Industry, National Engineering Research Center for Agrochemicals, Changsha 410014, China; 2. Hunan Province Key Laboratory for Agrochemicals, Changsha 410014, China) |
| Abstract：A HPLC method was established for the determination of methyl 2-((1-(4-chlorophenyl)-1H-pyrazol-3-yloxy)methyl)phenyl(hydroxy)carbamate. The HPLC system consisted of Aglient Exlend-C18 column(5 μm, 4.6 mm×150 mm), a mixture of methanol and 0.4% phosphoric acid aquas solution at a volume-ratio of 75 to 25 as mobile phase, 275 nm as the detected wavelength, a flow rate of 1mL/min and column temperature of 40℃. The experimental results showed that this method for the determination of methyl 2-((1-(4-chlorophenyl)-1H-pyrazol-3-yloxy)methyl)phenyl(hydroxy)carbamate had a good linear correlation in a rang from 0. 53 μg to 3.52 μg(y=1 256 794.4x+15 010.4; r=0.999 9). RSD was 0.24% and the recovery was in a rang from 99.2% to 100.4%. The complete period of analysis was 15 min. This method was proved to be sensitive, fast, and accurate. It could be applied in the quality control of methyl 2-((1-(4-chlorophenyl)-1H-pyrazol-3-yloxy)methyl)phenyl(hydroxy)carbamate. |
| Key words：methyl 2-((1-（4-chlorophenyl)-1H-pyrazol-3-yloxy)methyl)phenyl(hydroxy)carbamate |
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作者簡(jiǎn)介：孔曉紅（1972-），女，湖南長(cháng)沙人，助理研究員，主要從事農藥、精細化工中間體分析（E-mail：hehuayuan4602@sina.com）。
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收稿日期：2015-01-10
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