| 第 46 卷第 3 期 |  | Vol. 46 No. 3 | | 2016 年 6 月 | Jun 2016 |
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所屬欄目：醫藥及中間體
乙醛對假絲酵母脂肪酶催化活性的影響 |
| 魏英民，熊龍斌，付薪菱，徐 超，周建波*
（長(cháng)沙醫學(xué)院，湖南 長(cháng)沙 410219） |
| 摘 要：采用紫外可見(jiàn)分光光度法和熒光發(fā)射光譜法研究了乙醛溶液對假絲酵母脂肪酶水解三油酸甘油酯的催化活性和構象的影響。結果表明，低濃度乙醛能提高酶的催化水解活力，當乙醛濃度為0.221 5 mmol／L時(shí)，酶活力提高了18.84%；高濃度乙醛對酶活力有抑制作用，當乙醛濃度為3.322 5 mmol／L時(shí)，酶活力降低了25.85%。低濃度乙醛使酶催化反應的最適pH向堿性方向偏移，紫外吸收光譜和熒光發(fā)射光譜均有顯著(zhù)增強和光譜峰偏移現象。動(dòng)力學(xué)分析表明，加入乙醛后，酶的Vmax增大，Km減小。 |
| 關(guān)鍵詞：乙醛；假絲酵母脂肪酶；酶活性；光譜分析 |
| 中圖分類(lèi)號：TQ921 文獻標識碼：A 文章編號：1009-9212（2016）03-0028-04 |
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| Effect of Acetaldehyde on Catalytic Activity of Candida Rugosa Lipase |
| WEI Ying-min, XIONG Long-bin, FU Xin-ling, XU Chao, ZHOU Jian-bo*
(Changsha Medical University, Changsha 410219, China) |
| Abstract：The alteration of Candida rugosa lipase(CRL) activity and conformation were studied after pretreatment with acetaldehyde solutions at different concentrations by UV-vis spectroscopy and fluorescence emission spectroscopy. The results showed that the lipase activity increased in the presence of acetaldehyde at low concentration and the highest increasement was 18.84% when the acetaldehyde concentration was 0.221 5 mmol·L-1. The lipase activity decreased in the presence of acetaldehyde at high concentration and the highest decreasement was 25.85% when the acetaldehyde concentration was 3.322 5 mmol·L-1. The optimum pH value of the 1ipase in the presence of acetaldehyde increased by 0.5 compared to that in the absence of acetaldehyde. The UV absorption and fluorescence emission intensities of the lipase in the presence of acetaldehyde were enhanced markedly and the spectral peaks shifted conpared to the contrast. When CRL was treated with acetaldehyde, the Vmax increased while Km value declined． |
| Key words：acetaldehyde; Candida rugosa lipase; enzyme activity; spectrum analysis |
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基金項目：湖南省教育廳項目（14C0121和13C1127）。
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作者簡(jiǎn)介：魏英民（1985-），女，山東聊城人，碩士研究生，研究方向：酶催化（E-mail：weiym929@163.com）。
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聯(lián) 系 人：周建波，女，湖南衡陽(yáng)人，講師，研究方向：有機催化（E-mail：Zhoujianbo0603@126.com）。
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收稿日期：016-05-24
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