| 第 48 卷第 1 期 |  | Vol. 48 No. 1 | | 2018 年 2 月 | Feb 2018 |
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所屬欄目：醫藥及中間體
丹參-葛根提取物抗氧化能力與活性成分的相關(guān)性研究 |
| 肖 覃，楊思敏，胡 閩，劉琳琪*
（長(cháng)沙學(xué)院 生物與環(huán)境工程學(xué)院，湖南 長(cháng)沙 410022） |
| 摘 要：為揭示丹參-葛根提取物抗氧化作用的活性成分，以DPPH自由基清除率和總還原能力（FRAP）法評價(jià)提取物的抗氧化活性，建立UPLC分離方法鑒別其成分，采用Minitab相關(guān)性分析和偏最小二乘回歸法（PLS）分析提取物抗氧化能力與成分的相關(guān)性。結果表明，在提取物抗氧化性與單一成分相關(guān)性的研究中，大豆苷對抗氧化性的貢獻低。葛根素、丹酚酸B、迷迭香酸與DPPH清除率的相關(guān)性較強，相關(guān)系數R約為0.6，高于FRAP值的相關(guān)性（R約為0.5），說(shuō)明其主要通過(guò)清除自由基達到抗氧化作用。以葛根素、丹酚酸B、迷迭香酸、大豆苷4種組分為變量與DPPH清除率建立的關(guān)系模型中，R接近0.7，呈明顯的正相關(guān)，表明這四種組分是丹參-葛根提取物抗氧化活性的主要貢獻者。 |
| 關(guān)鍵詞：丹參；葛根；抗氧化；相關(guān)性 |
| 中圖分類(lèi)號：TQ464 文獻標識碼：A 文章編號：1009-9212（2018）02-0049-06 |
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| Study on the Correlation of Antioxidant Activity with Active Components Extracted from Salviae Miltiorrhizae and Radix Puerariae |
| XIAO Qin, YANG Si-min, HU Min，LIU Lin-qi*
(School of Biological and Environmental Engineering, Changsha University, Changsha 410022, China) |
| Abstract：DPPH free radical-scavenging and Ferric reducing antioxidant power (FRAP) assays were chosen to evaluate the antioxidant activities of salviae miltiorrhizae and radix puerariae extracts. The UHPLC method was established for the analysis of active components in salvia miltiorrhiza and radix puerariae. Minitab correlation analysis and partial least squares (PLS) were proposed for analysis of the correlation between antioxidant activities and component contents. Results showed that daidzein had low contribution to antioxidant capacity of extracts. The correlations of puerarin,salvianolic acid B, rosmarinic acid and DPPH free radical-scavenging was better. The coefficients of correlation(R) was 0.6, which was higher than the correlation coefficient(R=0.5) of Ferric reducing antioxidant power (FRAP) and activate components. It showed that the antioxidation effect was mainly achieved by scavenging free radicals. The model took four components daidzein, puerarin, salvianolic acid B and rosmarinic acid as independent variables, DPPH free radical-scavenging as variables was established. The correlation coefficient of the model approached 0.7, which presented significant positive correlation. It showed these four components are the main contributors for the antioxidant activity of extract from salvia miltiorrhiza and radix pueraria. The results established that scientific foundation for the development and application of antioxidants. |
| Key words：salviae miltiorrhizae; radix puerariae; antioxidant; correlation |
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基金項目：長(cháng)沙學(xué)院2017年大學(xué)生科技創(chuàng )新項目計劃。
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作者簡(jiǎn)介：肖 覃（1997-），女，湖南長(cháng)沙人，研究方向：植物活性成分的研究（E-mail：819636439@qq.com）。
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聯(lián) 系 人：劉琳琪，實(shí)驗師，研究方向：植物活性成分的研究（E-mail：linqi.liu@163.com）。
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收稿日期：2018-02-05
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