| 第 48 卷第 4 期 |  | Vol. 48 No. 4 | | 2018 年 8 月 | Aug 2018 |
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所屬欄目：分析與測試
丙硫唑高效液相色譜分析 |
| 廖麗萍1，2，3，陳 穎1，2，3，劉小鋒1，2，3
（1. 湖南化工研究院有限公司，國家農藥創(chuàng )制工程技術(shù)研究中心，湖南 長(cháng)沙 410014；2.農用化學(xué)品湖南省重點(diǎn)實(shí)驗室，湖南 長(cháng)沙 410014；3. 湖南化工研究院有限公司 湖南加法檢測有限公司，湖南 長(cháng)沙 410014） |
| 摘 要：建立丙硫唑的高效液相色譜分析方法。樣品用冰醋酸溶解，采用 Agilent TC-C18 反相柱為色譜柱，v（甲醇） ∶ v（水）=70 ∶ 30為流動(dòng)相，PDA檢測器在295 nm 對丙硫唑進(jìn)行高效液相色譜分離和測定。方法在32～320 μg·mL-1的濃度范圍內線(xiàn)性關(guān)系良好，其線(xiàn)性回歸方程：y=23 584x-517.84，相關(guān)系數r=0.999 9，變異系數為 0.2%，回收率為 99.0%～100.4%。該方法簡(jiǎn)單可靠，精確度和準確度符合定量分析要求，可用于丙硫唑定量分析。 |
| 關(guān)鍵詞：高效液相色譜；丙硫唑；分析 |
| 中圖分類(lèi)號：O657 文獻標識碼：A 文章編號：1009-9212（2018）04-0070-03 |
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| Determination of Methyl 5-(propylthio)-1H-benzo[d]imidazol-2-ylcarbamate by HPLC |
| LIAO Li-ping1,2,3,CHEN Ying1,2,3,LIU Xiao-feng1,2,3
(1. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry, Changsha 410014, China; 2. Hunan Province Key Laboratory for Agrochemicals, Changsha 410014, China; 3.HUNAN J & F TEST Co.,Ltd.， Changsha 410014，China) |
| Abstract：To establish a high performance liquid chromatography method for methyl 5-(propylthio)-1H-benzo[d]imidazol-2-ylcarbamate, the samples in glacial acetic acid was separated using reverse phase column Agilent TC-C18 as chromatographic column while mobile phase was used as a methanol to water volume ratio of 70:30. PDA detector was set to separate and read the signal of compound at 295 nm.This method detected compound's signal nicely and regressed to linear model between 32~320 μg·mL-1 of concentration. The regressed linear function was y=23 584x-517.84 and the regression coefficiency r was of 0.999 9, coefficient of variant is of 0.2%, recovery percentage was of 99.0% to 100.4%.The method reported here was a simple, stable and accurate one that meet the requirments for quantitative analysis of methyl 5-(propylthio)-1H-benzo[d]imidazol-2-ylcarbamate. |
| Key words：methyl 5-(propylthio)-1H-benzo[d]imidazol-2-ylcarbamate；analysis |
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作者簡(jiǎn)介：廖麗萍（1979-），女，湖南常寧人，研究方向：農藥、化工產(chǎn)品及農藥殘留的分析。
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收稿日期：2018-05-30
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