| 第 48 卷第 5 期 |  | Vol. 48 No. 5 | | 2018 年 10 月 | Oct 2018 |
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所屬欄目：安全與環(huán)保
嘧啶高效降解菌MD2的篩選、鑒定及其條件優(yōu)化 |
| 張海濤1，2，邱德躍1，2*，陳 燦1，2，秦岳軍1，2，張 燕1，2，馬 林1，2，劉 歡1，2，干興利1，2，鐘若楠1，2，柳愛(ài)平1，2*
（1. 湖南化工研究院有限公司，國家農藥創(chuàng )制工程技術(shù)研究中心，湖南 長(cháng)沙 410014；2. 農用化學(xué)品湖南省重點(diǎn)實(shí)驗室，湖南 長(cháng)沙 410014） |
| 摘 要：從實(shí)驗室嘧啶生化好氧池廢水及其活性污泥中分離得到1株耐鹽高效嘧啶降解菌MD2，該菌株可以嘧啶為唯一碳源生長(cháng)。通過(guò)形態(tài)特征、生理生化反應、16S rDNA鑒定及序列比分析，確定菌株MD2為1株檸檬酸桿菌；正交實(shí)驗確定MD2降解嘧啶的最佳環(huán)境條件組合為嘧啶濃度150 mg·L-1、鹽度1%、pH值7.0、溫度30℃，該條件下菌株MD2對嘧啶的降解率達到67.84%，比原來(lái)提高2.5%左右。 |
| 關(guān)鍵詞：嘧啶；檸檬酸桿菌；優(yōu)化；廢水處理 |
| 中圖分類(lèi)號：X703 文獻標識碼：A 文章編號：1009-9212（2018）05-0064-05 |
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| Isolation, Identification and Optimization of Pyrimidine-degrading Strain |
| ZHANG Hai-tao1,2, QIU De-yue1,2*, CHEN Can1,2, QIN Yue-jun1,2, ZHANG Yan1,2, MA Lin1,2, LIU Huan1,2, GAN Xing-li1,2, ZHONG Ruo-nan1,2, LIU Ai-ping1,2*
(1. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry, Changsha 410014, China; 2. Hunan Province Key Laboratory for Agrochemicals, Changsha 410014, China) |
| Abstract：One salt-tolerant and high-efficiency pyrimidine-degrading strain was isolated from the wastewater and activated sludge in the laboratory biochemical aerobic tank, which was used for pyrimidine wastewater treatment. The strain could feed pyrimidine as the sole carbon source and named MD2. According to morphological characteristics, physiological and biochemical reactions, 16S rDNA identification and sequence ratio analysis, strain MD2 was identified as a strain of Citrobacter sp.. The optimum environmental conditions for pyrimidine degradation by MD2 was obtained by orthogonal experiments. The pyrimidine concentration was 100 mg·L-1, the salinity was 1%, the pH was 7.0, and the temperature was 30℃. Under the above condition, the degradation rate of pyrimidine by strain MD2 reached 67.84%, about 2.5% higher than before. |
| Key words：pyrimidine; Citrobacter; optimization; wastewater treatment |
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基金項目：長(cháng)沙市科技計劃項目（kq1701173），環(huán)境友好與資源高效利用化工新技術(shù)，湖南省高校2011協(xié)同創(chuàng )新中心資助的成果。
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作者簡(jiǎn)介：張海濤（1991-），男，湖南邵陽(yáng)人，碩士，研究方向：環(huán)境污染修復與防治（E-mail 842692960@qq.com）。
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聯(lián) 系 人：邱德躍，副研究員，碩士，研究方向：廢水處理；柳愛(ài)平，研究員，博士，研究方向：新農藥創(chuàng )制。
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收稿日期： 2018-09-1
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