| 第 49 卷第 3 期 |  | Vol. 49 No. 3 | | 2019 年 6 月 | Jun 2019 |
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所屬欄目：分析與測試
三維熒光光譜法結合二階校正測定人體血清中的兩種抗精神病藥舒必利和氨磺必利 |
| 歐陽(yáng)洋子，吳海龍*，方 煥，孫小東，王 童，常月月，俞汝勤
（湖南大學(xué) 化學(xué)化工學(xué)院 化學(xué)生物傳感與計量學(xué)國家重點(diǎn)實(shí)驗室，湖南 長(cháng)沙 410082） |
| 摘 要：基于自加權交替三線(xiàn)性分解（SWATLD）算法，采用三維熒光結合二階校正方法同時(shí)快速檢測人血清樣中的兩種抗精神病藥舒必利（SPD）和氨磺必利（ASPD）。即使目標分析物之間、分析物與背景之間、以及分析物與其他未知干擾之間的光譜存在嚴重重疊現象，該策略仍能對數據進(jìn)行準確解析，并得到可靠滿(mǎn)意的定量預測結果。在人血清樣中，SPD 和ASPD 的平均回收率分別為（91.9±8.3）%和（91.7±10.8）%。此外，計算了四種品質(zhì)因子參數，如靈敏度（SEN）、選擇性（SEL）、檢測限（LOD）和定量限（LOQ），從而進(jìn)一步評估了該方法的性能。實(shí)驗結果表明，所提出的分析策略能夠用于臨床中人血清樣的SPD 和ASPD 的直接定量分析。 |
| 關(guān)鍵詞：三維熒光；二階校正；自加權交替三線(xiàn)性分解算法；舒必利；氨磺必利 |
| 中圖分類(lèi)號：O657.3 文獻標識碼：A 文章編號：1009-9212（2019）03-0063-06 |
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| Determination of Sulpiride and Amisulpiride in Human Serum Samples Using Excitation-emission Matrix Fluorescence Coupled with Second-order Calibra-tion Method |
| OUYANG Yang-zi, WU Hai-long*, FANG Huan, SUN Xiao-dong, WANG Tong, CHANG Yue-yue, YU Ru-qin
(State Key Laboratory of Chemo /Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China) |
| Abstract：A novel strategy was developed for simultaneously detecting sulpiride (SPD) and amisulpride (ASPD) in human serum samples using excitation-emission matrix fluorescence coupled with second-order calibration method based on the self-weighted alternating trilinear decomposition (SWATLD) algorithm. Even if there is a serious overlap between the targeted analytes, or among the targeted analytes and other unknown interferents, the strategy can accurately resolve the three-way data array and obtain the satisfactory analytical results as well. In human serum samples, the average recoveries of SPD and ASPD were (91.9 ± 8.3)% and (91.7 ± 10.8)%, respectively. In addition, four figures of merit such as sensitivity (SEN), selectivity (SEL), limit of detection (LOD), and limit of quantitation (LOQ) were calculated for further evaluating the accuracy of the proposed method. The analytical results indicated that the proposed strategy could be used for direct quantitative analysis of SPD and ASPD in clinical practice. |
| Key words：excitation-emission matrix fluorescence; second-order calibration; self-weighted alternating trilinear decomposition algorithm; sulpiride; amisulpiride |
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基金項目：國家自然科學(xué)基金資助項目（21575039，21775039）。
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作者簡(jiǎn)介：歐陽(yáng)洋子（1995-），女，湖南衡陽(yáng)人，碩士研究生，主要從事化學(xué)計量學(xué)多維校正理論及應用（E-mail: oy_yangzi@hnu.edu.cn）。
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聯(lián) 系 人：吳海龍，教授，主要從事化學(xué)計量學(xué)多維校正理論及應用相關(guān)研究（E-mail: hlwu@hnu.edu.cn）。
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收稿日期：2019-06-05
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