| 第 49 卷第 4 期 |  | Vol. 49 No. 4 | | 2019 年 8 月 | Aug 2019 |
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所屬欄目：醫藥及中間體
6-姜酚的制備與結構表征 |
| 凌 琳，李英奇，鄧珊霞，曾健青*
（湖南和廣生物科技有限公司，湖南 永州 425000） |
| 摘 要：以超臨界二氧化碳萃取所得的姜樹(shù)脂為原料，采用連續液-液萃取法制得6-姜酚粗品，再經(jīng)過(guò)加壓硅膠柱層析法，對其進(jìn)行分離純化，得到含量為95%以上的6-姜酚，通過(guò)質(zhì)譜與核磁共振波譜對其進(jìn)行結構表征�？疾煸现�6-姜酚含量、硅膠目數、操作溫度、洗脫溶劑流速等對6-姜酚柱層析分離效果的影響。結果表明，在層析硅膠目數為200~300目、操作溫度為10℃、洗脫溶劑的流速為1.35 mL·cm-2·min-1、6-姜酚粗品中6-姜酚含量為64.32%的條件下，進(jìn)行加壓柱層析，制備得到含量為95.12%的6-姜酚，6-姜酚的回收率81.46%。 |
| 關(guān)鍵詞：6-姜酚；姜樹(shù)脂；加壓硅膠柱層析法；結構表征 |
| 中圖分類(lèi)號：R284 文獻標識碼：A 文章編號：1009-9212（2019）04-0035-06 |
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| Preparation and Structural Characterization of 6-Gingerol |
| LING Lin, LI Ying-qi, DENG Shan-xia, ZENG Jian-qing*
(Hunan Health-Guard Biotechnology Incorporated Company, Yongzhou 425000, China) |
| Abstract：Using ginger oleoresin as the raw materia extracted from ginger by supercritical carbon dioxide extraction method, 6-gingerol with a purity of more than 95% was prepared efficiently through separation and purification by continuous liquid-liquid extraction and pressurized silica gel column chromatography. The obtained 6-gingerol was characterized by mass spectrometry and nuclear magnetic resonance spectroscopy. The effects of factors including the content of 6-gingerol in raw materials, the size of silica gels, operating temperature and the flow rate of elute on the separation efficiency of 6-gingerol were evaluated. The results showed that 6-gingerolwith a purity of 95.12% could be obtained rapidly with a yield of 81.47% under the following conditions: that the purity of 6-gingerol as raw material for pressurized silica gel column chromatography was of 64.32%; the size of silica gel was of 200~300 mesh; operating temperature was of 10℃ and the elute flow rate was of 1.35 mL·cm-2·min-1 for a gradient elution. |
| Key words：6-gingerol; ginger oleoresin; pressurized silica gel column chromatography; structural characterization |
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基金項目：湖南省創(chuàng )新創(chuàng )業(yè)技術(shù)投資項目（2017GK5038），湖南省企業(yè)科技創(chuàng )新創(chuàng )業(yè)團隊項目（和廣生物現代萃取分離與應用科技創(chuàng )新創(chuàng )業(yè)團隊）。
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作者簡(jiǎn)介：凌 琳（1991-），女，湖南株洲人，碩士，研究方向：植物提取、分離純化與應用（E-mail：18874848142@163.com）。
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聯(lián) 系 人：曾健青，博士，研究員，研究方向：超臨界流體技術(shù)研究與應用（E-mail：jackingzeng@vip.163.com）。
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收稿日期：2019-08-06
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