| 第 49 卷第 4 期 |  | Vol. 49 No. 4 | | 2019 年 8 月 | Aug 2019 |
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所屬欄目：分析與測試
柱前手性衍生化HPLC法拆分D，L-高絲氨酸內酯鹽酸鹽 |
| 解銀萍，王瀅秀，董文凱，柴洪偉*
（山東省農藥科學(xué)研究院 山東省重點(diǎn)實(shí)驗室，山東 濟南 250100） |
| 摘 要：建立了一種簡(jiǎn)單、快捷的柱前手性衍生高效液相色譜法拆分D，L-高絲氨酸內酯鹽酸鹽的方法。以鄰苯二甲醛（OPA）和N-乙�；�-L-半胱氨酸（NAC）為手性衍生化試劑，流動(dòng)相為乙腈-醋酸銨水溶液（v ∶ v=10 ∶ 90），色譜柱為WondaSil C18色譜柱，流速0.8 mL·min-1，柱溫25℃，在230 nm紫外檢測波長(cháng)下進(jìn)行檢測。L-高絲氨酸內酯鹽酸鹽與其光學(xué)異構體分離度大于3.5，該方法的線(xiàn)性相關(guān)系數為0.999 2，相對標準偏差為0.064%，加標回收率為99.25%。 |
| 關(guān)鍵詞：L-高絲氨酸內酯鹽酸鹽；柱前衍生；高效液相色譜；手性拆分 |
| 中圖分類(lèi)號：O657.7 文獻標識碼：A 文章編號：1009-9212（2019）04-0070-03 |
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| Chiral Separation of D,L-Homoserine Lactone Hydrochloride by HPLC with Chiral Pre-column Derivatization |
| XIE Yin-ping, WANG Ying-xiu, DONG Wen-kai, CHAI Hong-wei*
(Shandong Pesticide Research Institute, Shandong Province Key Laboratory of Chemical Pesticide, Jinan 250100, China) |
| Abstract：A simple and rapid chiral pre-colum derivation method for the optical purity of L-homoserine lactone hydrochloride was established. After derivatization with OPA and NAC, D, L-homoserine lactone hydrochloride was separated by HPLC. The chiral separation was carried out on a WondaSil C18 chromatographic column with a mixture of acetonitrile and ammonium acetate at a volume ratio of 10 ∶ 90 as mobile phase, a flow rate of 0.8 mL·min-1, a column temperature of 25℃ and the detection wave length of 230 nm. The results showed that the resolution factor was greater than 3.5 for L-homoserine lactone hydrochlorid and its enantiomer; the linear correlation coefficient was of 0.999 2; the standard deviation was of 0.064% and the average recovery was of 99.25%. |
| Key words：L-homoserine lactone hydrochloride; precolumn derivatization; HPLC; chiral separation |
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基金項目：山東省農業(yè)科學(xué)院農業(yè)科技創(chuàng )新工程（CXGC2016B12新型農藥創(chuàng )制關(guān)鍵技術(shù)）。
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作者簡(jiǎn)介：解銀萍（1988-），女，山東臨沂人，工程師，碩士研究生，研究方向：農藥合成工藝開(kāi)發(fā)（E-mail：xieyinping107@163.com）。
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聯(lián) 系 人：柴洪偉，工程師，研究方向：農藥合成工藝開(kāi)發(fā)（E-mail：hwchai@aliyum.com）。
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收稿日期：2019-08-19
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