| 第 49 卷第 6 期 |  | Vol. 49 No. 6 | | 2019 年 12 月 | Dec 2019 |
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所屬欄目：功能材料
均勻設計法優(yōu)化梔子苷類(lèi)活性成分提取工藝及其應用 |
| 姜 紅，向思孟，夏宇博，甘期望，劉琳琪*，趙晨曦
（長(cháng)沙學(xué)院 生物與環(huán)境工程學(xué)院，湖南 長(cháng)沙 410022） |
| 摘 要：以梔子苷的溶出率為指標，采用均勻設計U10×（108）考察了回流和超聲法中各提取條件對梔子苷類(lèi)活性成分溶出的影響，進(jìn)行回歸分析建立數學(xué)模型，得到優(yōu)化條件。結果表明超聲法明顯優(yōu)于回流法，確定了以70%乙醇按照20 mL·g-1液固比超聲提取50 min的提取條件。將該提取工藝用于10批梔子和10批焦梔子藥材的提取，結果發(fā)現，梔子藥材中梔子苷含量4.26%~6.29%。各批次焦梔子中梔子苷均低于梔子，含量在3.45%~5.23%。由此可見(jiàn)，采用均勻設計法可快速、準確地確定梔子苷類(lèi)活性成分的最佳提取工藝、條件，能更準確地評價(jià)和控制梔子藥材質(zhì)量。 |
| 關(guān)鍵詞：梔子；梔子苷；均勻設計；超聲波提��；高效液相色譜 |
| 中圖分類(lèi)號：R914.5 文獻標識碼：A 文章編號：1009-9212（2019）06-0049-05 |
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| Optimization and Application of Extraction Process of Iridoids Components from Gardeniae Fructus by Uniform Design |
| JIANG Hong, XIANG Si-meng, XIA Yu-bo, GAN Qi-wang, LIU Lin-qi*, ZHAO Chen-xi
(School of Biological and Environmental Engineering, Changsha University, Changsha 410022, China) |
| Abstract：In this paper, uniform design U10*(108) was used to investigate the factors affecting the release of iridoids components from Gardeniae Fructus in both reflux and ultrasonic extraction methods using the amount of gardenoside as evaluation criteria. The extraction conditions including time, solvent (ethanol) concentration and liquid-to-solid ratio were considered. Regression analysis were performed to establish mathematical models. The results showed that ultrasonic extraction method was obviously superior to the reflux extraction method. The optimal conditions for ultrasonic extraction process were obtained as following: 70% ethanol was used as solvent; the liquid-solid ratio was of 20 mL·g-1; and the extracting time was of 50 min. The extraction conditions were tested in extraction of 10 batches of Gardenia and 10 batches of coke Gardenia samples. The results showed that the iridoid glycosides constituents were different in different batches of gardenia medicinal materials. The contents of gardenoside varied from 4.26% to 6.29%. The contents of gardenoside in different batches of coke gardenia were lower than those in gardenia with a range of 3.45% to 5.23%. Thus, uniform design was successfully used to appraise the appropriate method and conditions for quick and accurate extraction of iridoid components. This presented a basis for quality evaluation and precise control of gardenia. |
| Key words：Gardeniae Fructus; Gardenoside; uniform design; ultrasonic extraction; HPLC |
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基金項目：湖南省自然科學(xué)基金項目（2017JJ3342），長(cháng)沙市科技計劃項目（kc1809036），2018年度長(cháng)沙學(xué)院“大學(xué)生創(chuàng )新項目計劃”，2019年度長(cháng)沙學(xué)院創(chuàng )新創(chuàng )業(yè)訓練計劃項目。
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作者簡(jiǎn)介：姜 紅（1999-），女，湖南寧鄉人，研究方向：植物活性成分的研究（E-mail：2644356131@qq.com）。
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聯(lián) 系 人：劉琳琪，實(shí)驗師，研究方向：植物活性成分的研究（E-mail：linqi.liu@163.com）。
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收稿日期：2019-11-27
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