| 第 50 卷第 1 期 |  | Vol. 50 No. 1 | | 2020 年 2 月 | Feb 2020 |
|
所屬欄目：功能材料
丹參-葛根藥對提取物的純化工藝及抗氧化活性研究 |
| 姜 紅，劉琳琪*，夏宇博，呂 超，趙晨曦
（長(cháng)沙學(xué)院 生物與環(huán)境工程學(xué)院，湖南 長(cháng)沙 410022） |
| 摘 要：為了將丹參-葛根藥對粗提物進(jìn)一步加工成抗氧化保健品，研究該粗提物的大孔樹(shù)脂純化工藝。以丹酚酸B、葛根素含量為指標，通過(guò)對九種大孔樹(shù)脂的靜態(tài)吸附量和解吸率的考察，篩選出合適的樹(shù)脂，優(yōu)化純化工藝。同時(shí)采用DPPH自由基法測定粗提物、純化物的抗氧化活性。結果表明：D101型大孔樹(shù)脂的純化工藝為：上樣濃度30 mg·mL-1，上樣量2 BV，除雜劑水用量為3 BV，洗脫劑乙醇濃度為50%，用量為3.5 BV。經(jīng)樹(shù)脂純化后，丹酚酸B和葛根素的含量分別提高了3.33倍、4.03倍，多糖含量明顯降低，丹參-葛根提取物清除DPPH自由基的IC50由0.048 3 mg·mL-1降至0.010 4 mg·mL-1，抗氧化能力顯著(zhù)增強。該純化工藝有效地提高了丹酚酸B和葛根素的含量，利于抗氧化保健品的研發(fā)。 |
| 關(guān)鍵詞：純化工藝；丹酚酸B；葛根素；大孔樹(shù)脂；抗氧化 |
| 中圖分類(lèi)號：R284.2 文獻標識碼：A 文章編號：1009-9212（2020）01-0055-06 |
|
| Purification and Antioxidant of Extracts from Salviae Miltiorrhizae and Radix Puerariae by Macroporous Resin |
| JIANG Hong, LIU Lin-qi*, XIA Yu-bo, LV Chao, ZHAO Chen-xi
(Department of Biological and Environmental Engineering, Changsha University, Changsha 410022, China) |
| Abstract：Antioxidant products were obtained using Salvia miltiorrhiza and Radix puerariae as the raw materials, the purification process of extracts by macroporous resin was studied. Monitoring the contents of salvianolic acid B and puerarin the appropriate resins were screened through the static adsorption capacity and desorption rate of 9 kinds of macroporous resins, and the purification process was optimized. The antioxidant activities of crude extracts and purified products were also determined by DPPH free radicals method. The optimized conditions of D101 macroporous resin were as followings: a crude drug concentration of Salviae miltiorrhizae-Radix puerariae of 30 mg·mL-1, a loading quantity of the sample of 2 BV, water as cleaning agent of 3 BV and 50% ethanol eluting phase of 3.5 BV. The content of salvianolic acid B and puerarin was increased by 3.33 times and 4.03 times, respectively after resin purification; polysaccharide was greatly reduced. Meanwhile, the IC50 of extracts from Salvia miltiorrhiza and Radix puerariae on the scavenging ability of DPPH radical decreased from 0.0483 mg·mL-1 to 0.010 4 mg·mL-1. It indicated that the antioxidant capacity of the purified extracts increased significantly. The purification process effectively increased the content of salvianolic acid B and puerarin, which is crucial to antioxidant product development. |
| Key words：purification process; salvianolic acid B; puerarin; macroporous resin; antioxidant |
|
基金項目：湖南省自然科學(xué)基金項目（2017JJ3342），長(cháng)沙市科技計劃項目（kc1809036），2018年度長(cháng)沙學(xué)院“大學(xué)生創(chuàng )新項目計劃”項目。
|
作者簡(jiǎn)介：姜 紅（1999-），女，湖南寧鄉人，研究方向：植物活性成分的研究（E-mail：2644356131@qq.com）。
|
聯(lián) 系 人：劉琳琪，實(shí)驗師，研究方向：植物活性成分的研究（E-mail：linqi.liu@163.com）。
|
收稿日期：2019-10-12
|
|