| 第 50 卷第 2 期 |  | Vol. 50 No. 2 | | 2020 年 4 月 | Apr 2020 |
|
所屬欄目：分析與測試
高效液相色譜-二極管陣列檢測器法測定青霉素亞砜酯含量 |
| 王云華，王志強，朱 君，畢 弋，冉兆晉，焦佳媛，柴寶山*
（沈陽(yáng)化工研究院有限公司，遼寧 沈陽(yáng) 110021） |
| 摘 要：建立高效液相色譜-二極管陣列檢測器法測定青霉素亞砜酯的含量。采用Agilent Eclipse Plus C18（3.0 mm×100 mm，3.5 μm）色譜柱；柱溫30 ℃；以25 mmol·L-1的醋酸銨水溶液（A）-乙腈（B）為流動(dòng)相進(jìn)行梯度洗脫；流速為0.5 mL·min-1；檢測波長(cháng)267 nm。青霉素亞砜酯在0.01~0.80 mg·mL-1（R2=0.999 9）范圍內線(xiàn)性較好，精密度RSD為0.15%。方法操作簡(jiǎn)單，結果準確，特異性強，穩定性好，適用于青霉素亞砜酯的含量測定。 |
| 關(guān)鍵詞：青霉素亞砜酯；高效液相色譜；二極管陣列檢測器；含量測定 |
| 中圖分類(lèi)號：O657.7+2 文獻標識碼：A 文章編號：1009-9212（2020）02-0067-03 |
|
| Determination of Penicillin Sulfoxide Ester by High Performance Liquid Chromatography Coupling with Photodiode Array Detector |
| WANG Yun-hua, WANG Zhi-qiang, ZHU Jun, BI Yi, RAN Zhao-jin, JIAO Jia-yuan, CHAI Bao-shan*
(Shenyang Research Institute of Chemical Industry Co.,Ltd., Shenyang 110021, China) |
| Abstract：A method was established for determination of penicillin sulfoxide ester by high performance liquid chromatography coupling with photodiode array detector. In this method Agilent Eclipse Plus C18(3.0×100 mm,3.5 μm) column was used and the temperature was set at 30 ℃. Gradient elution was performed with 25 mmol·L-1 ammonium acetate aqueous solution and acetonitrile as mobile phase. The flow rate was 0.5 mL·min-1 and the detection wavelength was 267 nm. The calibration curves of penicillin sulfoxide ester was linear in the range of 0.01~0.8 mg·mL-1(R2=0.999 9), and the RSD was 0.15%. This method is simple, accurate, highly specific and good stability, thus can be used for the determination penicillin sulfoxide ester. |
| Key words：penicillin sulfoxide ester; HPLC; DAD; content determination |
|
作者簡(jiǎn)介：王云華（1990-），女，遼寧沈陽(yáng)人，工程師，碩士，研究方向：藥物分析。
|
聯(lián) 系 人：柴寶山，高級工程師，博士，研究方向：藥物化學(xué)（E-mail：chaibaoshan@sinochem.com）。
|
收稿日期：2020-04-08
|
|