| 第 50 卷第 4 期 |  | Vol. 50 No. 4 | | 2020 年 8 月 | Aug 2020 |
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所屬欄目：醫藥及中間體
超臨界CO2萃取西藏黃瑪卡揮發(fā)油工藝優(yōu)化及GC-MS分析 |
| 薛世開(kāi)，朱東英，黃思宇，陳玉蓉，羅金花*
（長(cháng)沙學(xué)院 生物與環(huán)境工程學(xué)院，湖南 長(cháng)沙 410022） |
| 摘 要：研究了超臨界CO2萃取西藏黃瑪卡揮發(fā)油萃取工藝，并分析鑒定揮發(fā)油成分。以異氰酸芐酯、苯甲酸、2-苯乙酰胺、3-甲氧基苯乙腈、N-芐基甲酰胺含量為指標，以萃取壓力、萃取溫度和萃取時(shí)間為因素，設計單因素試驗和正交實(shí)驗。采用氣相-質(zhì)譜聯(lián)用（GC-MS）分析揮發(fā)油成分，色譜峰面積歸一化法計算各成分相對百分含量。優(yōu)化萃取工藝為：萃取溫度40 ℃，萃取壓力35 MPa，萃取時(shí)間2 h。通過(guò)質(zhì)譜庫檢索出35種化合物。該工藝穩定、可行，可用于萃取西藏產(chǎn)黃瑪卡揮發(fā)油。萃取工藝和成分分析結果可為西藏引種黃瑪卡資源及其質(zhì)量標準評價(jià)提供依據。 |
| 關(guān)鍵詞：瑪卡；揮發(fā)油；超臨界CO2萃��；化學(xué)成分；GC-MS |
| 中圖分類(lèi)號：O6 文獻標識碼：A 文章編號：1009-9212（2020）04-0024-06 |
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| Technology Optimization of Volatile Oil of Yellow Maca in Tibet by Supercritical CO2 Extraction and GC-MS Analysis |
| XUE Shi-kai, ZHU Dong-ying, HUANG Si-yu, CHEN Yu-rong, LUO Jin-hua*
(College of Biological and Environmental Engineering, Changsha University, Changsha 410022, China) |
| Abstract：To optimize the extraction technology by supercritical CO2 extraction and analyze the chemical composition of volatile oil of yellow Lepidium meyenii Walp. in Tibet. The contents of isocyanate benzyl ester, benzoic acid, 2-phenyl ethyl amide, 3-methoxy benzene acetonitrile, N-benzyl formamide were taken as the indices, extraction pressure, extraction temperature and extraction time were taken as factors to design single factor experiment and orthogonal experiment. Gas chromatography-mass spectrometry (GC-MS) combined with retention index were used to analyze the composition of volatile oil and the relative contents of the components were determinated by area normalization. The optimum extraction condition were the extraction temperature of 40 ℃, the extraction pressure of 35 MPa, and the extraction time of 2 h; and 35 compounds were identified by mass spectrometry. This technology is stable and feasible, and can be used to extract the volatile oil of Lepidium meyenii Walp. in Tibet. The extraction technology and component analysis results provide scientific basis for the extracting Lepidium meyenii Walp in Tibet and its quality standard evaluation. |
| Key words：Lepidium meyenii Walp.; volatile oil; supercritical CO2 extraction; chemical composition; GC-MS |
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基金項目：湖南省自然科學(xué)基金青年項目（2017JJ3340），湖南省教育廳一般項目（17C0127），長(cháng)沙學(xué)院博士科研啟動(dòng)基金項目（SF1603）。
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作者簡(jiǎn)介：薛世開(kāi)（2001—），男，湖南益陽(yáng)人，主要從事植物精油綜合性能的研究（E-mail：1729799255@qq.com）。
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聯(lián) 系 人：羅金花，女，講師，博士，主要從事理論計算及天然產(chǎn)物研究（E-mail：luojinhua9988@163.com）。
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收稿日期：2020-07-01
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