第 32 卷第 6 期 |  | Vol. 32 No. 6 | 2002 年 12 月 | Dec 2002 |
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所屬欄目:分析與測試
1,4-二巰基蘇醇的高效液相色譜分析 |
高永民; 李輝; 陳美玲; 王樹(shù)偉(河北威遠集團; 石家莊金魚(yú)涂料集團; 050031; 050051) |
摘 要:采用C18 反相柱和紫外吸收檢測器 ,以甲醇 +乙腈 +水作流動(dòng)相 ,對 1,4 二巰基蘇醇進(jìn)行液相色譜分離 ,外標法定量。方法線(xiàn)性范圍寬、精密度好、準確度高。 |
關(guān)鍵詞:1; 4二巰基蘇醇; 液相色譜; 分析 |
中圖分類(lèi)號: 文獻標識碼: 文章編號:1009-9212(2002)06-0054-01 |
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Analysis of 1, 4-Dithiothreitol by HPLC |
GAO Yong-min; et al.(Hebei Vian Group; Shijiazhuang; 050031; China) |
Abstract:The quantitative analysis of 1,4-Dithiothreitol by HPLC on C 18 reversed phase column with UV-absorbance detector and mobile phase of methol + acetonitrile + water. The calibration curve was linear in the range of 0.01~2.0 g/L, the standard deviation was 0.19, the coefficient of variation was 0.20% and the recovery was 97.8%~102.0%. |
Key words:Dithiothreitol; HPLC; analysis |
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收稿日期:2002-01-21
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