| 第 51 卷第 4 期 |  | Vol. 51 No. 4 | | 2021 年 8 月 | Aug 2021 |
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所屬欄目：分析與測試
三維熒光結合二階校正法同時(shí)測定人體液中的兩種抗丙型肝炎藥物 |
| 童高焱，吳海龍*，常月月，王 童，諶露珠，俞汝勤
（湖南大學(xué) 化學(xué)化工學(xué)院 化學(xué)生物傳感與計量學(xué)國家重點(diǎn)實(shí)驗室，湖南 長(cháng)沙 410082） |
| 摘 要：提出一種三維熒光結合基于交替三線(xiàn)性分解（ATLD）算法的二階校正方法快速對人體血清和尿液中的兩種抗丙型肝炎藥物達卡他韋（DAC）和雷迪帕韋（LED）進(jìn)行定性定量的分析方法。二階校正方法因具有“二階優(yōu)勢”，可避免復雜的樣本預處理，即使分析物之間和分析物與未知干擾之間存在嚴重的光譜重疊，該方法仍可得到滿(mǎn)意的定性定量結果。兩種藥物的平均回收率為98.35%~109.95%，相關(guān)系數大于0.99。同時(shí)，相應的品質(zhì)因子參數反映出該方法具有較高的靈敏度，較好的選擇性和較低的檢測限。這一方法的建立有望為臨床藥物的快速分析提供新思路。 |
| 關(guān)鍵詞：三維熒光；二階校正；交替三線(xiàn)性分解；二階優(yōu)勢；丙型肝炎 |
| 中圖分類(lèi)號：R969 文獻標識碼：A 文章編號：1009-9212（2021）04-0054-07 |
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| Simultaneous Determination of Two Anti-HCV Drugs in Human Fluids Using Three-way Fluorescence Coupled with Second-order Calibration Method |
| TONG Gao-yan, WU Hai-long*, CHANG Yue-yue, WANG Tong, CHEN Lu-zhu, YU Ru-qin
(State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China) |
| Abstract：This work proposed an analytical method that three-way fluorescence coupled with second-order calibration based on alternative trilinear decomposition (ATLD) algorithm to rapidly and simultaneously quantify two anti-hepatitis C drugs including daclatasvir (DAC) and ledipasvir (LED) in human serum and urine. Using the "second-order advantage" of the second-order calibration method can avoid complicated sample pretreatment. Even if the fluorescence spectra of interested analytes overlapped and unknown interference coexisted in the system, the method can still require satisfactory qualitative and quantitative results. The average recoveries were 98.35%~109.95%, and the correlation coefficients were more than 0.99. The figures of merit also showed that the method has higher sensitivity and better selectivity and lower limit of detection. The establishment of this method is expected to provide new ideas for the rapid determination of clinical drugs. |
| Key words：three-way fluorescence; second-order calibration; alternative trilinear decomposition; second-order advantage; hepatitis C |
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基金項目：國家自然科學(xué)基金資助項目（21775039，2217040266，21521063）。
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作者簡(jiǎn)介：童高焱（1996-），女，云南昭通人，碩士研究生，主要從事化學(xué)計量學(xué)多維校正理論及應用（E-mail：gytong@hnu.edu.cn）。
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聯(lián) 系 人：吳海龍，教授，主要從事化學(xué)計量學(xué)多維校正理論及應用相關(guān)研究（E-mail：hlwu@hnu.edu.cn）。
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收稿日期：2021-08-23
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