| 第 51 卷第 4 期 |  | Vol. 51 No. 4 | | 2021 年 8 月 | Aug 2021 |
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所屬欄目：分析與測試
吡噻菌胺及其代謝物在番茄中殘留分析方法研究 |
| 孫 炯，任 競*，傅 強，付啟明，馮 雪，何 鑫，楊 柳，周詩(shī)瑜
（湖南文譜檢測技術(shù)研究有限公司，湖南 長(cháng)沙 410137） |
| 摘 要：采用QuEChERS前處理方法結合高效液相色譜-串聯(lián)質(zhì)譜（UPLC-MS／MS）多反應監測（MRM）模式測定吡噻菌胺及其代謝物1-甲基-3-（三氟甲基）-1H-吡唑-4-甲酰胺（PMA）在番茄中的殘留量。樣品經(jīng)0.2%甲酸乙腈提取，N-丙基乙二胺（PSA）和無(wú)水硫酸鎂凈化，UPLC-MS／MS檢測。方法在0.000 1~0.6 mg／L范圍內有良好的線(xiàn)性關(guān)系，番茄中吡噻菌胺和1-甲基-3-（三氟甲基）-1H-吡唑-4-甲酰胺（PMA）最低檢出量均為3×10-13 g，定量限為0.002 mg／kg。樣本中添加量為0.002、0.01、0.1、1.0、2.0 mg／kg時(shí)（n=5），平均回收率92.6%~104.8%，相對標準偏差1.3%~2.7%。 |
| 關(guān)鍵詞：吡噻菌胺；PMA；QuECh ERS；高效液相色譜-串聯(lián)質(zhì)譜；殘留 |
| 中圖分類(lèi)號：O657.7 文獻標識碼：A 文章編號：1009-9212（2021）04-0061-04 |
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| Analytic Method for the Residue of Penthiopyrad and Its Metabolite PMA in Tomata |
| SUN Jiong, REN Jing*, FU Qiang, FU Qi-ming, FENG Xue, HE Xin, YANG Liu, ZHOU Shi-yu
(Hunan Winp Analytical Technology Research Co.,Ltd., Changsha 410137, China) |
| Abstract：The residues of Penthiopyrad and its metabolite PMA in tomato were investigated by QuEChERS-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)(MRM). Samples were prepared through extraction using solvent of 0.2% formic acid in acetonitrile, and purified by both PSA and MgSO4, were analyzed by UPLC-MS/MS. The linear range of the method was from 0.000 1 to 0.6 mg/L with a determination coefficient (R2) of more than 0.99. The limit of detection (LOD) for both Penthiopyrad and PMA was of 3×10-13 g, and the limit of quantitation (LOQ) was of 0.002 mg/kg. At the four levels including 0.002, 0.01, 0.1, 1.0 and 2.0 mg·kg-1, the average recovery of the penthiopyrad and PMA was between 92.6% and 104.8%, the RSD were between 1.3% and 2.7%. |
| Key words：Penthiopyrad; PMA; QuEChERS; UPLC-MS/MS; detecting residues |
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作者簡(jiǎn)介：孫 炯（1984-）男，湖南寧鄉人，碩士，研究方向：農藥殘留檢測、農藥質(zhì)量檢測（E-mail：sunjiong2010@163.com）。
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聯(lián) 系 人：任競，助理研究員，碩士，研究方向：農藥殘留分析（E-mail: renjing-1980@sina.cn）。
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收稿日期：2021-05-27
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