| 第 51 卷第 5 期 |  | Vol. 51 No. 5 | | 2021 年 10 月 | Oct 2021 |
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所屬欄目：分析與測試
環(huán)丙氟蟲(chóng)胺原藥高效液相色譜分析 |
| 吳 若1，肖瑛子1，胡金蕾1，唐 亮1，王 丹1，龐懷林2
（1. 長(cháng)沙嘉橋生物科技有限公司，湖南 長(cháng)沙410209；2. 南通泰禾化工股份有限公司，江蘇 南通 226407） |
| 摘 要：研究了高效液相色譜測定環(huán)丙氟蟲(chóng)胺原藥含量的HPLC方法。采用C18不銹鋼色譜柱（150 mm×4.6 mm i.d.× 5 μm）和紫外檢測器，以甲醇-0.1%磷酸水溶液（V∶V=75∶25）為流動(dòng)相，流速為1.5 mL／min，在240 nm波長(cháng)下，柱溫為40 ℃±2 ℃，進(jìn)樣體積為10 μL。在上述色譜操作條件下，對環(huán)丙氟蟲(chóng)胺原藥進(jìn)行了定量分析�？疾炝朔治龇椒ㄌ禺愋�、線(xiàn)性回歸方程、精密度和準確度。該方法測得環(huán)丙氟蟲(chóng)胺的保留時(shí)間約8.6 min，線(xiàn)性相關(guān)系數為0.999 8，標準偏差0.10，變異系數0.10%，平均回收率99.20%。 |
| 關(guān)鍵詞：環(huán)丙氟蟲(chóng)胺；高效液相色譜；分析 |
| 中圖分類(lèi)號：O657.7+2 文獻標識碼：A 文章編號：1009-9212（2021）05-0093-03 |
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| Analysis of Cyproflanilide TC by HPLC |
| WU Ruo1, XIAO Ying-zi1, HU Jin-lei1, TANG Liang1, WANG Dan1, PANG Huai-lin2
(1. Changsha Jia-qiao Biotech Co.,Ltd., Changsha 410209, China；2. CAC Nantong Chemicals Co., Ltd., Nantong 226407, China) |
| Abstract：The content of Cyproflanilide was determined using HPLC. In this method, the sample was analyzed using a stainless steel column (150 mm×4.6 mm i.d.×5 μm) packed with C18 filler equipped with a UV detector of 240 nm; a mixture of methanol and 0.1% phosphoric acid aqueous solution was used as mobile phase at a flow rate of 1.5 mL/min. The factors including specificity, linear regression equation, precision and accuracy of the method were investigated. Ruselts showed that the retention time of Cyproflanilide was 8.6 min； the linear correlation was of 0.999 8；a standard deviation was of 0.10; a variation coefficient was of 0.10%, and an average recovery of 99.20% were obtained. The results showed that this method was sensitive, simple and rapid, and suitable for the quantitative analysis of cyproflanilide. |
| Key words：cyproflanilide; HPLC; analysis |
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作者簡(jiǎn)介：吳 若（1987—），男，安徽安慶人，研究方向：農藥分析方法的研究及標準編寫(xiě)（E-mail：ruo_wu@cacch.com）
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收稿日期：2021-08-03
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