| 第 52 卷第 4 期 |  | Vol. 52 No. 4 | | 2022 年 8 月 | Aug 2022 |
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所屬欄目：分析與測試
氣相色譜法測定和厚樸酚原料藥中殘留溶劑的研究 |
| 陳莉莉1，楊云霞2，門(mén) 靖3
（1. 陜西大河藥業(yè)有限責任公司，陜西 西安 710200；2. 陜西省藥品和疫苗檢查中心咸陽(yáng)分中心，陜西 咸陽(yáng) 712000；3. 西安萬(wàn)隆制藥股份有限公司，陜西 西安 710119） |
| 摘 要：采用氣相色譜法，以二甲基亞砜為溶劑，頂空進(jìn)樣，以DB-624毛細管柱程序升溫，FID檢測器，建立了和厚樸酚中乙醇、二氯甲烷、正己烷等殘留溶劑的測定方法。結果表明：乙醇在2.500 4~750.120 μg/mL范圍線(xiàn)性方程為y=0.800 9x+4.133 2，相關(guān)系數0.999 1，線(xiàn)性良好；二氯甲烷在2.422~90.825 μg/mL范圍線(xiàn)性方程y=0.894 2x-0.639，相關(guān)系數0.999 5，線(xiàn)性良好；正己烷在0.096 469~43.845 μg/mL范圍線(xiàn)性方程為y=42.072x-29.422，相關(guān)系數0.995 5，線(xiàn)性良好。所建立的方法靈敏度高，準確性好，操作簡(jiǎn)便，適用于和厚樸酚殘留溶劑的測定。 |
| 關(guān)鍵詞：和厚樸酚；殘留溶劑；頂空進(jìn)樣；氣相色譜法 |
| 中圖分類(lèi)號：R284.1 文獻標識碼：A 文章編號：1009-9212（2022）04-0084-05 |
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| Determination of Residual Solvents in Honokiol by Gas Chromatography |
| CHEN Li-li1, YANG Yun-xia2, MEN Jing3
(1. Shaanxi Dahe Pharmaceutical Co., Ltd., Xi’an 710200, China; 2. Xianyang Branch of Shaanxi Province Drug and Vaccine Inspection Center, Xiangyang 712000, China; 3. Xianyang Wanlong Pharmaceutical Co., Ltd., Xi’an 710119, China) |
| Abstract：In a gas chromatography method, using the dimethyl sulfoxide as solvent, adopting the headspace sampling method, raising the temperature through DB-624 capillary column program, FID as the detector, the residual solvent such as ethanol, dichlormethane and n-hexane in honokiol was detected. The results showed that: the linear equation was expressed as y=0.800 9x+4.133 2 when ethanol is in the range of 2.500 4~750.120 μg/mL, the correlation coefficient reached 0.999 1, showing good linearity. The linear equation was expressed as y=0.894 2x-0.639 when dichloromethane falled in the range of 2.422~90.825 ug/mL, the correlation coefficient reached 0.999 5, with good linearity. And it was expressed as y=42.072x-29.422 when n-hexane falled in the range of 0.096 469~43.845 μg/mL, also showing the correlation coefficient of 0.995 5 and good linearity. This method was suitable for the determination of honokiol and honokiol residual solvents with the advantages including high sensitivity, good accuracy and simple operation. |
| Key words：honokiol; residual solvent; headspace sampling; gas chromatography method |
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作者簡(jiǎn)介：陳莉莉（1980—），女，陜西蒲城人，主管藥師，執業(yè)藥師，研究方向：醫藥研發(fā)、植物提取及中藥化學(xué)研究（E-mail：695766755@qq.com）。
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收稿日期：2022-04-19
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