| 第 52 卷第 5 期 |  | Vol. 52 No. 5 | | 2022 年 10 月 | Oct 2022 |
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所屬欄目：分析與測試
二氯異丙蟲(chóng)酰胺的高效液相色譜分析 |
| 劉小鋒1，2，3，陳 穎1，2，3，陳一萍1，2，3
（1. 湖南化工研究院有限公司 國家農藥創(chuàng )制工程技術(shù)研究中心，湖南 長(cháng)沙 410014；2. 農用化學(xué)品湖南省重點(diǎn)實(shí)驗室，湖南 長(cháng)沙 410014；3. 湖南化研院檢測技術(shù)有限公司，湖南 長(cháng)沙 410014） |
| 摘 要：建立了二氯異丙蟲(chóng)酰胺的高效液相色譜分析方法。樣品用乙腈溶解，采用Agilent TC-C18柱為色譜柱，V（乙腈）∶V（水）=50∶50為流動(dòng)相，PDA檢測器在245 nm對二氯異丙蟲(chóng)酰胺進(jìn)行高效液相色譜分離和檢測。方法在13.6~680 µg/mL的濃度范圍內線(xiàn)性關(guān)系良好，線(xiàn)性回歸方程為y=22 659x-15 086，相關(guān)系數R2=0.999 9，變異系數為0.37，平均回收率為99.9%。該方法簡(jiǎn)單可靠，精密度及準確度符合定量分析要求，可用于二氯異丙蟲(chóng)酰胺的定量分析。 |
| 關(guān)鍵詞：高效液相色譜；二氯異丙蟲(chóng)酰胺；分析 |
| 中圖分類(lèi)號：O657.7+2 文獻標識碼：A 文章編號：1009-9212（2022）05-0088-03 |
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| Determination of 3-Bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(isopropylcarbamoyl)phenyl)-1H-pyrazole-5-carboxamide by HPLC |
| LIU Xiao-feng1,2,3, CHEN Ying1,2,3, CHEN Yi-ping1,2,3
(1. National Engineering Reserch Center for Agrochemicals, Hunan Research Institute of Chemical Industry Co., Ltd., Changsha 410014, China; 2. Hunan Province Key Laboratory for Agrochemicals, Changsha 410014, China; 3. Hunan Chemical Research Institute Testing Technology Co., Ltd., Changsha 410014, China) |
| Abstract：To establish a high performance liquid chromatography method for 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(isopropylcarbamoyl)phenyl)-1H-pyrazole-5-carboxamide, the samples dissolved in acetonitrile were separated using reverse phase column Agilent TC-C18 as chromatographic column with a mobile phase of acetonitrile to water volume ratio of 50∶50; PDA detector was selected to separate and read the signal of compound at 245 nm. This method could obtain compound’s signal nicely with a linear model between 13.6~680 µg/mL of concentration. The regressed linear was y=22 659x-15 086 and the regression coefficiency R² was of 0.999 9; the coefficient of variant was of 0.37; the average recovery was of 99.9%. This method was simple, stable and accurate, and meet the requirements for quantitative analysis of 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(isopropylcarbamoyl)phenyl)-1H-pyrazole-5-carboxamide. |
| Key words：HPLC; 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(isopropylcarbamoyl)phenyl)-1H-pyrazole-5-carboxamide; analysis |
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作者簡(jiǎn)介：劉小鋒（1993—），男，湖南邵東人，研究方向：農藥分析（E-mail：411388486@qq.com）。
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收稿日期：2022-09-01
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