| 第 53 卷第 2 期 |  | Vol. 53 No. 2 | | 2023 年 4 月 | Apr 2023 |
|
所屬欄目：分析與測試
LC-MS液質(zhì)聯(lián)用法測定白酒中甜蜜素的含量 |
| 周遠明，胡景田，陳貴蘭，王倩文*
（青島農業(yè)大學(xué) 分析測試中心，山東 青島 266109） |
| 摘 要：白酒樣品用超純水直接稀釋?zhuān)捎?0 mmoL/L乙酸銨水溶液和甲醇作為流動(dòng)相，梯度洗脫，液質(zhì)聯(lián)用法測定白酒中甜蜜素含量。結果表明，甜蜜素質(zhì)量濃度在0.7~14 μg/mL范圍內線(xiàn)性關(guān)系良好（R2=0.995，n=6），檢測限和定量限分別為0.6 μg/kg及3 μg/kg，精密度實(shí)驗相對標準偏差為9.7%，回收率在70%以上。該方法樣品前處理簡(jiǎn)單，可以減少污染風(fēng)險，分析過(guò)程快捷準確，重復性好，可用于白酒中甜蜜素的含量測定。 |
| 關(guān)鍵詞：白酒；甜蜜素；液質(zhì)聯(lián)用法 |
| 中圖分類(lèi)號：O656.3 文獻標識碼：A 文章編號：1009-9212（2023）02-0079-05 |
|
| Content Determination of Sodium Cyclamate in Liquor by High Performance Lliquid Chromatography-tandem Mass Spectrometry |
| ZHOU Yuan-ming, HU Jing-tian, CHEN Gui-lan, WANG Qian-wen*
(Analytical and Testing Center, Qingdao Agricultural University, Qingdao 266109, China) |
| Abstract：Liquor sample was directly diluted with ultrapure water, liquid chromatography-tandem mass spectrometry method was used to determine sodium cyclamate content in liquor with methanol and 10 mmoL/L ammonium acetate water solution as mobile phase for gradient elution. The results show that the linear range of sodium cyclamate was 0.7~14 μg/mL (R2=0.995, n=6)．The limit of detection and limit of quantitation of sodium cyclamate were 0.6 μg/kg and 3 μg/kg, respectively. The RSD value was 9.7%, the recovery rate was above 70%. This method has the advantages of simple sample pretreatment with the reduction of the sample contamination likelihood, fast analysis process and fine repetition. So this method could be used for determination of the sodium cyclamate content in liquor. |
| Key words：liquor; sodium cyclamate; liquid chromatography-tandem mass spectrometry |
|
基金項目：青島農業(yè)大學(xué)高層次人才科研基金（663/1119027）。
|
作者簡(jiǎn)介：周遠明（1974—），男，湖北隨州人，高級實(shí)驗師，博士，研究方向：天然藥物化學(xué)（E-mail：zym7410@163.com）。
|
聯(lián) 系 人：王倩文，高級實(shí)驗師，博士，研究方向：儀器分析方法的開(kāi)發(fā)與研究（E-mail：wangqianwen_ac@126.com）。
|
收稿日期：2023-01-13
|
|