| 第 54 卷第 1 期 |  | Vol. 54 No. 1 | | 2024 年 2 月 | Feb 2024 |
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所屬欄目：醫藥及中間體
酶法頭孢拉定母液冷凍濃縮提取工藝研究 |
| 郭永剛1，劉 華2，張立斌3，4，5*，任 峰3，4，5，王利杰3，4，5，張建麗3，4，5
（1. 河北省藥品職業(yè)化檢查員總隊（南片區），河北 石家莊 050000；2. 河北省藥品職業(yè)化檢查員總隊（北片區），河北 秦皇島 066100；3. 華北制藥河北華民藥業(yè)有限責任公司，河北 石家莊 052165；4. 抗生素酶催化與結晶技術(shù)國家地方聯(lián)合工程實(shí)驗室，河北 石家莊 052165；5. 河北省頭孢類(lèi)藥物制備技術(shù)創(chuàng )新中心，河北 石家莊 052165） |
| 摘 要：以頭孢拉定離心母液為研究對象，研究了冷凍濃縮提取頭孢拉定工藝，考察了冷凍裝置、冷凍溫度、pH、冷凍時(shí)間及攪拌方式等因素對冷凍濃縮效果的影響。結果表明：采用內刮式冷凍濃縮裝置進(jìn)行冷凍濃縮，冷凍溫度-8 ℃，冷凍時(shí)間4 h，攪拌轉速150 r/min時(shí)，冷凍濃縮效果較好，頭孢拉定提取率88.7%。 |
| 關(guān)鍵詞：頭孢拉定；母液；冷凍濃縮 |
| 中圖分類(lèi)號：TQ460.6 文獻標識碼：A 文章編號：1009-9212（2024）01-0037-04 |
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| Study on the Freeze Concentration Process of Enzymatic Cefradine Mother Liquor |
| GUO Yong-gang1, LIU Hua2, ZAHNG Li-bin3,4,5*, REN Feng3,4,5, WANG Li-jie3,4,5, ZHANG Jian-li3,4,5
(1. Hebei Pharmaceutical Professional Inspector Corps (South Region), Shijiazhuang 050000, China; 2. Hebei Pharmaceutical Professional Inspector Corps (North Region), Qinhuangdao 066100, China; 3. NCPC Hebei Huamin Pharmaceutical Co., Ltd., Shijiazhuang 052165, China; 4. National and Local Joint Engineering Laboratory of Antibiotic Enzyme Catalysis and Crystallization Technology, Shijiazhuang 052165, China; 5. Hebei Province Cephalosporide Medicine Preparation Technology Innovation Center, Shijiazhuang 052165, China) |
| Abstract：To conduct experimental research on freeze concentration, cefradine centrifuge mother liquor was used as the research object. The effects of factors incuding freezing device, freezing temperature, pH value, freezing time, and stirring mode on the freezing process were studied. The results showed that using an internal scraping type freeze concentration device for freeze concentration, at a freezing temperature of -8 ℃, a freezing time of 4 hours, and a stirring speed of 150 r/min, the better freeze concentration effect was achieved, and the extraction yield of cefradine was 88.7%. |
| Key words：cefradine; mother solution; freeze concentration |
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作者簡(jiǎn)介：郭永剛（1982—），男，河北石家莊人，碩士，研究方向：藥物化學(xué)合成研究（E-mail：13780300521@163.com）。
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聯(lián) 系 人：張立斌，高級工程師，研究方向：抗生素類(lèi)藥物合成及產(chǎn)業(yè)化研究（E-mail：119783274@qq.com）。
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收稿日期：2023-12-01
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