| 第 33 卷第 1 期 |  | Vol. 33 No. 1 | | 2003 年 2 月 | Feb 2003 |
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所屬欄目：分析與測試
高效液相色譜法分析復配除草劑中芐嘧磺隆和丁草胺 |
| 石國榮; 劉惠言; 姜輝霞; 劉祥華(湖南農業(yè)大學(xué)理學(xué)院; 長(cháng)沙石油廠(chǎng); 湖南長(cháng)沙; 410128; 410008) |
| 摘 要：采用反相C18柱和紫外檢測器 ,對丁·芐可濕性粉劑中芐嘧磺隆和丁草胺的測定方法進(jìn)行了研究。結果表明 ,以甲醇 (A)和醋酸 -醋酸鈉緩沖溶液 (B ,pH =5.6 )為流動(dòng)相,采用梯度淋洗:0～5.5min ,A∶B =65∶35 (v/v) ,5.6min后 ,改為A∶B =85∶15 (v/v) ;在波長(cháng)254nm下檢測 ,芐嘧磺隆和丁草胺的變異系數分別為 1.20%和 0.83% ,平均回收率分別為99.4 %和 99.9% ,芐嘧磺隆和丁草胺的濃度分別在 0.001～0.360mg/mL和 0.015～7.740mg/mL的范圍內線(xiàn)性關(guān)系良好。方法準確、快速、重現性好 ,已用于丁·芐可濕性粉劑的質(zhì)量控制。 |
| 關(guān)鍵詞：芐嘧磺隆; 丁草胺; 高效液相色譜 |
| 中圖分類(lèi)號：TQ457.2 文獻標識碼：A 文章編號：1009-9212(2003)01-0052-02 |
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| Separation and Determination of Bensulfuron and Butachlor by High Performance Liquid Chromatography |
| SHI Guo-Rong; et al.（College of Science; Hunan Agricultural University; Changsha; 410128; China） |
| Abstract：A reversed-phase high performance liquid chromatographic method for the separation and determination of bensulfuron and butachlor in the composite herbicde has been developed. The sample was separated on phenomenex C 18 column 250mm×4mm i.d, methanol (A) and HAc-NaAc buffer solution (B,pH=5.6) as mobile phase use gradient elution, 0～5.5min, A∶B=65∶35(v/v), then change to A∶B=85∶15(v/v) after 5.6mins; It was then detected at 254nm by DAD detector. Quantification was carried out with the two external sta... |
| Key words：bensulfuron; butachlor; high performance liquid chromatography |
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基金項目：湖南農業(yè)大學(xué)青年科研基金項目(2001-012)
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收稿日期：2002-10-03
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