| 第 35 卷第 2 期 |  | Vol. 35 No. 2 | | 2005 年 4 月 | Apr 2005 |
|
所屬欄目：分析與測試
α-氰基-3-苯氧基芐醇的HPLC分離與測定 |
| 羅積杏; 唐世平; 楊立榮(上海市農藥研究所; 浙江大學(xué)生物工程研究所; 200032; 浙江杭州; 310027) |
| 摘 要：利用手性色譜柱SumichiralOA4400,及流動(dòng)相正己烷:二氯乙烷:乙醇=87∶10∶3(v/v),在流速1.0ml·min1,檢測波長(cháng)254nm,柱溫15℃的分析條件下,成功實(shí)現了對映體(氰基3苯氧基芐醇的手性分離和定量分析,(S)異構體和(R)異構體的線(xiàn)性相關(guān)系數分別為0.9981和0.9975,變異系數分別為0.63%和0.64%,回收率分別為98.7%～101.3%和99.1%～99.9%。 |
| 關(guān)鍵詞：α-氰基-3-苯氧基芐醇; 高效液相色譜; 分離; 測定 |
| 中圖分類(lèi)號：T255 文獻標識碼：A 文章編號：1009-9212(2005)02-0071-03 |
|
| The separation and determination of α-cyano-3-phenoxybenzyl alcohol by HPLC |
| LUO Ji-xing ~（1）; TANG Shi-ping~（2）; YANG Li-rong~（2）（1.Shanghai Pesticide Research Institute; Shanghai 200032; China; 2.Institute of Bioengineering; Zhejiang University; Hangzhou 310027; China） |
| Abstract：Using normal HPLC module, α-cyano-3-phenoxy benzyl alcohol was excellent separated and its isomers were quantitative determined on a Sumichiral OA-4400 column, using n-hexane, 1,2-Dichloroethane and ethanol with volume rate 87∶10∶3 as mobile phase, under optimized conditions : detection wavelength 254 nm, mobile phase flow rate 1.0ml·min~(-1), and column temperature 15℃. The variation coefficients of the(S)- and(R)-enantiomer were 0.63% and 0 .64%, and the linear correlations were 0.9981 and 0.9975, the rec... |
| Key words：α-cyano-3-phenoxybenzyl alcohol; HPLC; separation; determination |
|
收稿日期：2004-03-26
|
| 閱讀全文 |
|