| 第 31 卷第 4 期 |  | Vol. 31 No. 4 | | 2001 年 8 月 | Aug 2001 |
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所屬欄目：分析與測試
苯基熒光酮熒光法測定Sn~(2+)H_2O_2體系產(chǎn)生的·OH |
| 任風(fēng)蓮; 吳南; 黃樹(shù)琨; 吳心傳(中南工業(yè)大學(xué)化工學(xué)院; 湖南大學(xué)化學(xué)系; 湖南教育學(xué)院) |
| 摘 要：提出了一種快速、簡(jiǎn)便地測定Sn(Ⅱ ) -H2 O2 體系產(chǎn)生羥自由基 (·OH)的新方法。用熒光法建立了測定羥自由基的產(chǎn)生量 ,采用苯基熒光酮 phenylfluorone作為Sn(Ⅱ ) -H2 O2 反應體系的熒光試劑。通過(guò)測定苯基熒光酮反應前后的熒光強度變化 ,間接地測定羥自由基產(chǎn)生量。找到了體系最佳實(shí)驗條件。利用電子自旋共振法ERS ,測定苯基熒光酮加入前后的波譜變化 ,從而進(jìn)一步證明了該方法的可靠性。同時(shí) ,測定了幾種保健藥物對羥自由基 (·OH)的清除能力。方法簡(jiǎn)便 ,測定快速 ,穩定性好 ,靈敏度高 ,無(wú)需昂貴的儀器 ,可作為一種篩選抗氧化劑的簡(jiǎn)便方法 ,亦可作為食品保鮮 ,化妝品性能檢驗的一種方法。 |
| 關(guān)鍵詞：羥自由基; 熒光法; 苯基熒光酮; 過(guò)氧化氫; SnCl2 |
| 中圖分類(lèi)號：O657.3 文獻標識碼：A 文章編號：1009-9212(2001)04-0048-03 |
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| Phenyfluorene Fluoremetry Assay of Hydroxyl Radical Produced by Sn~(2+)H_2O_2 |
| REN Feng lian 1; WU Nan 1; HUANG Shu kun 2; WU Xin chuan 3 （1. Department of chenistry; Central South University of Technology; Changsha 410083; China; 2. Department of Chemistry; University of Hu Nan Changsha 410081; 3. Department of Chm |
| Abstract：It was found that the yield of Hydroxyl radicals produced in Sn 2+ -H 2O 2 system was 100 times more than that produced in Fenton reaction.A fluorimetry method for the determination of hydroxyl radicals was developed based Phenyfluorene Sn 2+ H 2O 2 system.The excitation and emission wavelength were chosen at 500nm and 510nm.With measurement of fluorescence intensity, the content of hydroxyl radicals in the solution was successfully determined.The results of clearing expericent and ESR showed ... |
| Key words：Phenylfluorene; Hydroxyl radical; Hydrogen peroxide; SnCl_2 |
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收稿日期：2000-11-13
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